GTF2IRD1 Monoclonal antibody

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  WB analysis using 68899-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68899-1-Ig (GTF2IRD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

GTF2IRD1 Monoclonal Antibody for WB, ELISA

Cat No. 68899-1-Ig

Clone No.1E5H3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Synonyms

GTF3, GTF2I repeat domain-containing protein 1, General transcription factor II-I repeat domain-containing protein 1, CREAM1, BEN

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, hTERT-RPE1 cells, HeLa cells, TT cells, MKN-45 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68899-1-Ig targets GTF2IRD1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10761

  Product name: Recombinant human GTF2IRD1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 610-959 aa of BC018136

  Sequence: ISLRRPNCFGIAKLRKILEASNSIQFVIKRPELLTEGVKEPIVDSQGTASSLGFSPPALPPERDSGDPLVDESLKRQGFQENYDARLSRIDIANTLREQVQDLFNKKYGEALGIKYPVQVPYKRIKSNPGSVIIEGLPPGIPFRKPCTFGSQNLERILAVADKIKFTVTRPFQGLIPKPDEDDANRLGEKVILREQVKELFNEKYGEALGLNRPVLVPYKLIRDSPDAVEVTGLPDDIPFRNPNTYDIHRLEKILKAREHVRMVIINQLQPFAEICNDAKVPAKDSSIPKRKRKRVSEGNSVSSSSSSSSSSSSNPDSVASANQISLVQWPMYMVDYAGLNVQLPGPLNY

• Full Name: GTF2I repeat domain containing 1
• Calculated Molecular Weight: 959 aa, 106 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: BC018136
• Gene Symbol: GTF2IRD1
• Gene ID (NCBI): 9569
• RRID: AB_3670449
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UHL9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.