GYG1 Recombinant antibody

• 

  WB analysis using 86056-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86056-1-RR (GYG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86056-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86056-1-RR against Human GYG1 were performed. The affinity constant is 0.115 nM.

GYG1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86056-1-RR

Clone No.250590C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:2.4.1.186, glycogenin 1, Glycogenin-1, GN1, GN-1

---

Tested Applications

• Positive WB detected in: HeLa cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86056-1-RR targets GYG1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GYG1 fusion protein Ag3583
• Full Name: glycogenin 1
• Calculated Molecular Weight: 350 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC031096
• Gene Symbol: GYG1
• Gene ID (NCBI): 2992
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P46976
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The GYG1 gene encodes glycogenin-1, a core protein in muscle glycogen particles. GYG1 is a glycosyltransferase that forms a short glucose polymer of approximately 10 glucose residues by autoglucosylation (PMID: 25272951 ). Cardiomyopathy and muscle weakness associated with muscle glycogen depletion can also be caused by impaired priming of glycogen synthesis due to inactivation of muscle glycogenin (glycogenin-1) (PMID: 20357282).