Galectin-3 Polyclonal antibody

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  WB analysis using 32664-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32664-1-AP (Galectin-3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 32664-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32664-1-AP (Galectin-3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 32664-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32664-1-AP (Galectin-3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 32664-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using Galectin-3 antibody (32664-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Galectin-3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 32664-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Gal-3, Lgals3, 35 kDa lectin

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, NIH/3T3 cells, mouse spleen tissue
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32664-1-AP targets Galectin-3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: lectin, galactose binding, soluble 3
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 30 kDa
• Gene Symbol: Lgals3
• Gene ID (NCBI): 16854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P16110
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosaccharides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which it can form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in the nucleus and cytoplasm or secreted outside the cell, on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).