Glypican 6 Polyclonal antibody

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  WB analysis using 21526-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21526-1-AP (Glypican 6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Glypican 6 Polyclonal Antibody for WB, ELISA

Cat No. 21526-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

GPC6, Secreted glypican-6, UNQ369/PRO705

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Tested Applications

• Positive WB detected in: HEK-293 cells, L02 cells, MG-63 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21526-1-AP targets Glypican 6 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16135

  Product name: Recombinant human GPC6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 213-278 aa of BC106947

  Sequence: RAFIAARTFVQGLTVGREVANRVSKVSPTPGCIRALMKMLYCPYCRGLPTVRPCNNYCLNVMKGCL

• Full Name: glypican 6
• Calculated Molecular Weight: 555 aa, 63 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC106947
• Gene Symbol: GPC6
• Gene ID (NCBI): 10082
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y625
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glypican-6 (GPC6) is a glycosylphosphatidylinositol-anchored heparan-sulfate proteoglycan that regulates cell-surface signaling cues during development and disease. GPC6 binds Hedgehog ligands and facilitates their interaction with the receptor Patched, thereby amplifying Hh signaling required for long-bone and gastric/intestinal elongation. (PMID: 35235423)