ChromoTek HA-Peptide

Validation Data Gallery

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  The HA-Trap Agarose was used to immunoprecipitate HA-PCNA fusion protein from HEK293T cells. HA-PCNA protein was released from the trap through a two-step competitive elution with HA-peptide. Samples from the Input (I), Flow-Through (F), 1st elution (E1), 2nd elution (E2), and residual (R) fractions were analyzed through WB. PCNA Monoclonal Antibody (60097-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001) were used in the WB analysis. Note: PCNA forms trimers resulting in co-elution of endogenous PCNA proteins with HA-tagged PCNA.

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  The HA-Trap Magnetic Agarose was used to immunoprecipitate HA-PCNA fusion protein from HEK293T cells. HA-PCNA protein was released from the trap through a two-step competitive elution utizling HA-peptide. Samples from the Input (I), Flow-Through (F), 1st elution (E1), 2nd elution (E2), and residual (R) fractions were analyzed through WB. PCNA Monoclonal Antibody (60097-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001) were used in the WB analysis. Note: PCNA forms timers resulting in co-elution of endogenous PCNA proteins with HA-tagged PCNA.

• 

  The HA-Trap Magnetic Particles M-270 was used to immunoprecipitate HA-PCNA fusion protein from HEK293T cells. HA-PCNA protein was released from the trap through a two-step competitive elution utizling HA-peptide. Samples from the Input (I), Flow-Through (F), 1st elution (E1), 2nd elution (E2), and residual (R) fractions were analyzed through WB. PCNA Monoclonal Antibody (60097-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001) were used in the WB analysis. Note: PCNA forms timers resulting in co-elution of endogenous PCNA proteins with HA-tagged PCNA.

HPLC-purified HA-peptide

Cat No. ap

Peptide

HA Tag

Applications

Elution

Conjugate

Unconjugated

Synonyms

HA peptide, hemagglutinin

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Product Information

HPLC-purified HA-peptide

• Description: HPLC-purified HA-peptide
• Applications: Elution of HA-tagged fusion proteins bound to HA-Traps (ata, atma, atd)
• Sequence: YPYDVPDYA
• Form: Lyophilized Powder
• Conjugate: Unconjugated
• Molecular Weight: 1.1 kDa
• Purity: 95%
• Reconstitution: Reconstitute peptide in 1550 uL PBS, which results in a final concentration of 500 µM. Vortex for 1 min to dissolve powder.
• Storage Condition: Upon receipt store at -20°C. Aliquot upon reconstitution and store at -20°C. Avoid repeated freeze/thaw cycles.
• Stability: Stable for 1 year upon receipt
• Shipment: Shipped at ambient temperature

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