HB9 Polyclonal antibody

HB9 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20975-1-AP

Synonyms

HB9, HLXB9, Homeobox protein HB9, HOXHB9, MNX1, SCRA1

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Validation Data Gallery

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  WB analysis of mouse spleen using 20975-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 20975-1-AP (HB9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20975-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20975-1-AP (HB9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20975-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20975-1-AP (HB9 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse spleen tissue, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20975-1-AP targets HB9 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: motor neuron and pancreas homeobox 1
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: NM_005515
• Gene Symbol: HB9
• Gene ID (NCBI): 3110
• RRID: AB_2878779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Motor neuron and pancreas homeobox (MNX1) is a homologous box transcription factor that promotes motor neuron differentiation and pancreatic development. MNX1 knockout in mice indicated that only the early expression of MNX1 is associated with the formation of pancreatic buds, and MNX1 is expressed in late β cells and associated with β cell maturation . MNX1 has the ability to induce endocrine progenitor cells to differentiate into β cells and inhibit their ability to differentiate into α cells, thereby maintaining the balance between α and β cells. In addition, the association between MNX1 gene mutations and Currarino syndrome has also been demonstrated. The Currarino syndrome phenotype caused by a MNX1 mutations was demonstrated to be a result of haploinsufficiency . A recent study also indicated that MNX1 may be an oncogene in prostate cancer (PMID: 30066929).