HCN1 Polyclonal antibody, PBS Only

• 

  WB analysis of mouse brain using 55222-1-AP (same clone as 55222-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55222-1-AP (HCN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55222-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 55222-1-AP (same clone as 55222-1-PBS)

  IP result of anti-HCN1 (IP:55222-1-AP, 4ug; Detection:55222-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 55222-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 55222-1-AP (same clone as 55222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 55222-1-AP (HCN1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55222-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cerebellum using 55222-1-AP (same clone as 55222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55222-1-AP (HCN1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 55222-1-PBS in a different storage buffer formulation.

HCN1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 55222-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, HAC 2, Brain cyclic nucleotide-gated channel 1, BCNG-1

---

Product Information

55222-1-PBS targets HCN1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: hyperpolarization activated cyclic nucleotide-gated potassium channel 1
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 99-120 kDa
• GenBank Accession Number: NM_021072
• Gene Symbol: HCN1
• Gene ID (NCBI): 348980
• RRID: AB_11182929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60741
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HCN1, also named as BCNG1, belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. HCN1 contributes to the native pacemaker currents in heart (If) and in neurons (Ih). It is activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. HCN1 may mediate responses to sour stimuli.