HCN1 Polyclonal antibody

HCN1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55222-1-AP

Synonyms

HAC 2, HCN1



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman heart tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

55222-1-AP targets HCN1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name hyperpolarization activated cyclic nucleotide-gated potassium channel 1
Calculated Molecular Weight 99 kDa
Observed Molecular Weight 99-120 kDa
GenBank Accession NumberNM_021072
Gene Symbol HCN1
Gene ID (NCBI) 348980
RRIDAB_11182929
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HCN1, also named as BCNG1, belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. HCN1 contributes to the native pacemaker currents in heart (If) and in neurons (Ih). It is activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. HCN1 may mediate responses to sour stimuli.

Protocols

Product Specific Protocols
WB protocol for HCN1 antibody 55222-1-APDownload protocol
IHC protocol for HCN1 antibody 55222-1-APDownload protocol
IP protocol for HCN1 antibody 55222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Affect Disord

Prenatal alcohol exposure enhanced alcohol preference and susceptibility to PTSD in a sex-dependent manner through the synaptic HCN1 channel

Authors - Hui Yao
humanWB

iScience

Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia

Authors - Yu-Han H Hsu
mouseWB

Cell Rep

Optogenetic Activation of Striatopallidal Neurons Reveals Altered HCN Gating in DYT1 Dystonia.

Authors - Giuseppe Sciamanna
mouseWB

Neurosci Bull

Thalamocortical Circuit Controls Neuropathic Pain via Up-regulation of HCN2 in the Ventral Posterolateral Thalamus

Authors - Yi Yan