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  • KD/KO Validated

HDAC8 Antibody

HDAC8 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 17548-1-AP

Synonyms

HD8, HDAC8, HDACL1, histone deacetylase 8, RPD3



Tested Applications

Positive WB detected inHeLa cells, Raji cells
Positive IP detected inRaji cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17548-1-AP targets HDAC8 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDAC8 fusion protein Ag11696
Full Name histone deacetylase 8
Calculated molecular weight 377aa,42 kDa
Observed molecular weight 49 kDa
GenBank accession number BC050433
Gene symbol HDAC8
Gene ID (NCBI) 55869
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone deacetylases (HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 8 was primarily found in the nucleus. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. This antibody is a rabbit polyclonal antibody raised against full length HDAC8 of human origin.

Protocols

Product Specific Protocols
WB protocol for HDAC8 antibody 17548-1-APDownload protocol
IF protocol for HDAC8 antibody 17548-1-APDownload protocol
IP protocol for HDAC8 antibody 17548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Res

Stabilized peptide HDAC inhibitors derived from HDAC1 substrate H3K56 for the treatment of cancer stem-like cells in vivo.

Authors - Dongyuan Wang
humanWB

Biol Open

HDAC3 and HDAC8 are required for cilia assembly and elongation.

Authors - Seon-Ah Park
  • KD Validated
mouseWB

Chem Res Toxicol

Curcumin Derivative Epigenetically Reactivates Nrf2 Antioxidative Stress Signaling in Mouse Prostate Cancer TRAMP C1 Cells.

Authors - Wenji Li
humanWB

Chem Res Toxicol

Epigenetics reactivation of Nrf2 in Prostate TRAMP C1 Cells by curcumin analog FN1.

Authors - Wenji Li
humanWB

FEBS Lett

Downregulation of histone deacetylase 1 by microRNA-520h contributes to the chemotherapeutic effect of doxorubicin.

Authors - Shen Qi Q
humanWB

Mol Carcinog

The triterpenoid corosolic acid blocks transformation and epigenetically reactivates Nrf2 in TRAMP-C1 prostate cells.

Authors - Jie Yang