HIGD2A Polyclonal antibody

HIGD2A Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21415-1-AP

Synonyms

HIG1 domain family member 2A, HIGD2A

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Validation Data Gallery

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  WB analysis of mouse kidney using 21415-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21415-1-AP (HIGD2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 21415-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HIGD2A antibody (21415-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

21415-1-AP targets HIGD2A in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HIGD2A fusion protein Ag15892
• Full Name: HIG1 hypoxia inducible domain family, member 2A
• Calculated Molecular Weight: 106 aa, 12 kDa
• Observed Molecular Weight: 11 kDa, 50 kDa
• GenBank Accession Number: BC007502
• Gene Symbol: HIGD2A
• Gene ID (NCBI): 192286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian HIGD-family proteins include two subgroups of yeast Rcf1 homologs, HIGD1A/B/C and HIGD2A/B. HIGD1A and HIGD2A (of 10.4 and 11.5 kDa, respectively) have two hydrophilic transmembrane domains and are localized in the inner mitochondrial membrane. HIGD1A and HIGD2A expression is induced under stress conditions like hypoxia or glucose deprivation in human cervical epithelial cells and murine cerebral cortical neurons. HIGD1A may play pleiotropic roles in mitochondrial respiratory chain biogenesis. HIGD2A forms a 50 kDa regulatory module with COX4-1, COX5B, and COX6A1, which binds to newly synthesized COX3 to promote its binding to the previously assembled COX1 + COX2 module (PMID:33291261,32375044).

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV; Authors - Daniella H Hock ; KO Validated
human	WB	Mol Cell Proteomics; HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV.; Authors - Daniella H Hock