Tested Applications
| Positive WB detected in | mouse kidney tissue |
| Positive IHC detected in | mouse brain tissue, human gliomas tissue, human renal cell carcinoma tissue, mouse heart tissue, mouse kidney tissue, rat brain tissue, rat heart tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
55408-1-AP targets HIPK2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | homeodomain interacting protein kinase 2 |
| Calculated Molecular Weight | 131 kDa |
| Observed Molecular Weight | 131 kDa, 101 kDa |
| GenBank Accession Number | NM_022740 |
| Gene Symbol | HIPK2 |
| Gene ID (NCBI) | 28996 |
| RRID | AB_2881323 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H2X6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HIPK2 is a serine/threonine kinase participating in transcriptional regulation, growth control, and apoptosis. The kinase contributes to the regulation of remarkably diverse pathways such as p53 activation or Wnt signaling. There is very low levels of HIPK2 protein expression in several thyroid tumors and the combination of HIPK2 and Gal-3,would be helpful in ameliorating the preoperative recognition of thyroid cancer. HIPK2 may represent a new tumour suppressor gene for these types of cancers and may constitute a new potential promising diagnostic marker and therapeutic target.(PMID:21698151).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HIPK2 antibody 55408-1-AP | Download protocol |
| WB protocol for HIPK2 antibody 55408-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Chem Biol Interact HIPK2 overexpression relieves hypoxia/reoxygenation-induced apoptosis and oxidative damage of cardiomyocytes through enhancement of the Nrf2/ARE signaling pathway.
| ||
Cell Res Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate | ||
Cancer Biol Ther Exosome-transmitted hsa_circ_0012634 suppresses pancreatic ductal adenocarcinoma progression through regulating miR-147b/HIPK2 axis | ||
J Hazard Mater Polystyrene nanoplastics induce cardiotoxicity by upregulating HIPK2 and activating the P53 and TGF-β1/Smad3 pathways |
