Histone H2B Polyclonal antibody

Histone H2B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 27740-1-AP


H2B clustered histone 26, H2B type 12, H2B3B, H2BC26, HIST3H2BB, histone cluster 3, H2bb, Histone H2B type 3 B

Tested Applications

Positive WB detected inCOLO 320 cells, HEK-293T cells, HEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27740-1-AP targets Histone H2B in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Histone H2B fusion protein Ag26901
Full Name histone cluster 3, H2bb
Observed Molecular Weight15-20 kDa
GenBank Accession NumberBC100855
Gene Symbol HIST3H2BB
Gene ID (NCBI) 128312
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST3H2BB is a replication-dependent histone that is a member of the histone H2B family.


Product Specific Protocols
WB protocol for Histone H2B antibody 27740-1-APDownload protocol
Standard Protocols
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