HLA-F Monoclonal antibody

HLA-F Monoclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1









Cat no : 66819-1-Ig


CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen, HLAF, HLA-F, Leukocyte antigen F, MHC class I antigen F

Tested Applications

Positive WB detected inA431 cells, NCCIT cells, Raji cells, human peripheral blood leukocytes
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRaji cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:8000-1:32000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66819-1-Ig targets HLA-F in WB, FC, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HLA-F fusion protein Ag6475
Full Name major histocompatibility complex, class I, F
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC062991
Gene Symbol HLA-F
Gene ID (NCBI) 3134
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)


Product Specific Protocols
WB protocol for HLA-F antibody 66819-1-IgDownload protocol
IHC protocol for HLA-F antibody 66819-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols