HLA class I (HLA-B) Polyclonal antibody, PBS Only

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  WB analysis using 17260-1-AP (same clone as 17260-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 17260-1-AP (same clone as 17260-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 17260-1-AP (same clone as 17260-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 17260-1-AP (same clone as 17260-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 17260-1-AP (same clone as 17260-1-PBS)

  IP result of anti-HLA class I (HLA-B) (IP:17260-1-AP, 4ug; Detection:17260-1-AP 1:4000) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 17260-1-AP (same clone as 17260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 17260-1-AP (same clone as 17260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 17260-1-AP (same clone as 17260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 17260-1-AP (same clone as 17260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 17260-1-AP (same clone as 17260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 17260-1-AP (same clone as 17260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 17260-1-AP (same clone as 17260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 17260-1-AP (same clone as 17260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 17260-1-AP (same clone as 17260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 17260-1-AP (same clone as 17260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 17260-1-AP (same clone as 17260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 17260-1-AP (same clone as 17260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 17260-1-AP (same clone as 17260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 17260-1-AP (same clone as 17260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary using 17260-1-AP (same clone as 17260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 17260-1-AP (same clone as 17260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 17260-1-AP (same clone as 17260-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HLA class I (HLA-B) antibody (17260-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17260-1-PBS in a different storage buffer formulation.

HLA class I (HLA-B) Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 17260-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

HLA-B, HLA B, HLA class I histocompatibility antigen, B alpha chain, Human leukocyte antigen B, MHC class I antigen

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Product Information

17260-1-PBS targets HLA class I (HLA-B) in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10947

  Product name: Recombinant human HLA class I (HLA-B) protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-362 aa of BC013187

  Sequence: MRVTAPRTVLLLLSGALALTETWAGSHSMRYFYTAMSRPGRGEPRFISVGYVDDTQFVRFDSDAASPREEPRAPWIEQEGPEYWDRNTQICKTNTQTYRESLRNLRGYYNQSEAGSHTLQRMYGCDVGPDGRLLRGHDQYAYDGKDYIALNEDLSSWTAADTAAQITQRKWEAAREAEQLRAYLEGLCVEWLRRYLENGKETLQRADPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEPSSQSTIPIVGIVAGLAVLAVVVIGAVVATVMCRRKSSGGKGGSYSQAASSDSAQGSDVSLTA

• Full Name: major histocompatibility complex, class I, B
• Calculated Molecular Weight: 362 aa, 40 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC013187
• Gene Symbol: HLA class I (HLA-B)
• Gene ID (NCBI): 3106
• RRID: AB_1939610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01889
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. This polyclonal antibody raised against human HLA-B can also react with HLA-A and HLA-C. (PMID: 667938; 3375250)