Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, Jurkat cells, MCF-7 cells, HeLa cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human stomach cancer tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human spleen tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17260-1-AP targets HLA class I (HLA-B) in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10947 Product name: Recombinant human HLA class I (HLA-B) protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-362 aa of BC013187 Sequence: MRVTAPRTVLLLLSGALALTETWAGSHSMRYFYTAMSRPGRGEPRFISVGYVDDTQFVRFDSDAASPREEPRAPWIEQEGPEYWDRNTQICKTNTQTYRESLRNLRGYYNQSEAGSHTLQRMYGCDVGPDGRLLRGHDQYAYDGKDYIALNEDLSSWTAADTAAQITQRKWEAAREAEQLRAYLEGLCVEWLRRYLENGKETLQRADPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEPSSQSTIPIVGIVAGLAVLAVVVIGAVVATVMCRRKSSGGKGGSYSQAASSDSAQGSDVSLTA Predict reactive species |
| Full Name | major histocompatibility complex, class I, B |
| Calculated Molecular Weight | 362 aa, 40 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC013187 |
| Gene Symbol | HLA class I (HLA-B) |
| Gene ID (NCBI) | 3106 |
| RRID | AB_1939610 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01889 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. This polyclonal antibody raised against human HLA-B can also react with HLA-A and HLA-C. (PMID: 667938; 3375250)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HLA class I (HLA-B) antibody 17260-1-AP | Download protocol |
| IHC protocol for HLA class I (HLA-B) antibody 17260-1-AP | Download protocol |
| IP protocol for HLA class I (HLA-B) antibody 17260-1-AP | Download protocol |
| WB protocol for HLA class I (HLA-B) antibody 17260-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HLA class I (HLA-B) Polyclonal antibody (Cat# 17260-1-AP) has 10 citations spanning journals including Signal Transduction and Targeted Therapy, Gut, Cell Death & Disease, Investigative Ophthalmology & Visual Science, PNAS Nexus, Cell Signaling, ACS Chemical Biology, Braz J Otorhinolaryngol, eLife, and bioRxiv. Research fields encompass oncology (lung, breast, liver, pancreatic, nasopharyngeal cancers), immunology (HLA/immunopeptidome), virology (HBV, Influenza A), and cell biology (extracellular vesicles, retinal endothelial cells).
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Sintilimab plus concurrent chemoradiotherapy for treatment of locally advanced small cell lung cancer (SINCE-01): a phase II clinical trial. | ||
Gut Single-cell RNA sequencing reveals intrahepatic and peripheral immune characteristics related to disease phases in HBV-infected patients. | ||
Cell Death Dis Exosomal annexin A6 induces gemcitabine resistance by inhibiting ubiquitination and degradation of EGFR in triple-negative breast cancer. | ||
Invest Ophthalmol Vis Sci Response of Human Retinal Microvascular Endothelial Cells to Influenza A (H1N1) Infection and the Underlying Molecular Mechanism | ||
PNAS Nexus Secreted HLA-Fc fusion profiles immunopeptidome in hypoxic PDAC and cellular senescence | ||
Cell Signal Inhibition of SIRT7 promotes STAT1 activation and STAT1-dependent signaling in hepatocellular carcinoma. |
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application. The reviewer confirmed that the antibody recognizes a specific band at the expected molecular weight, indicating reliable performance and specificity for its intended use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (09-16-2020) | This antibody recognises a specific band at the expected MW.
|
