HMBS Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 87431-1-RR (same clone as 87431-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87431-1-RR (HMBS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87431-1-PBS in a different storage buffer formulation.

• 

  Affinity of 87431-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87431-1-RR against Human HMBS were performed. The affinity constant is below 1 pM.

HMBS Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87431-1-PBS

Clone No.252812E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

EC:2.5.1.61, hydroxymethylbilane synthase, PBG D, PBGD, PBG-D

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Product Information

87431-1-PBS targets HMBS in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6509

  Product name: Recombinant human HMBS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-361 aa of BC000520

  Sequence: MSGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAMSTTGDKILDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILDVARQLNDAH

• Full Name: hydroxymethylbilane synthase
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC000520
• Gene Symbol: HMBS
• Gene ID (NCBI): 3145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08397
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Hydroxymethylbilane synthase (HMBS), also known as porphobilinogen deaminase (PBGD) or previously as uroporphyrinogen I synthase, is a key enzyme in the heme biosynthesis pathway. HMBS is a cytoplasmic enzyme that catalyzes a critical step in the heme biosynthesis pathway: the polymerization of four porphobilinogen molecules to form linear hydroxymethylbilane. It is expressed in all tissues, with the highest levels observed in the liver and erythroid precursors to meet high heme demand. Two main isoforms exist: a ubiquitous "housekeeping" isoform and an erythroid-specific isoform. The observed molecular weight of human HMBS protein is approximately 40-42 kDa (as determined by SDS-PAGE). The erythroid-specific isoform has a slightly lower molecular weight due to a different translation initiation site. (PMID: 19292878, PMID: 12555854)