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HMGB1 Polyclonal antibody
HMGB1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 10829-1-AP
|Positive WB detected in||Jurkat cells, RAW 264.7 cells, human brain tissue, rat stomach tissue, , rat brain tissue, human testis tissue, mouse kidney tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||mouse brain tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10829-1-AP targets HMGB1 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HMGB1 fusion protein Ag1264|
|Full Name||high-mobility group box 1|
|Calculated molecular weight||25 kDa|
|Observed molecular weight||30 kDa|
|GenBank accession number||BC003378|
|Gene ID (NCBI)||3146|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling (23649808).This antibody is a rabbit polyclonal antibody raised against full length HMGB1 of human origin. The monomeric HMGB1 is 29 kDa and dimer is 58 kDa.
|Product Specific Protocols|
|WB protocol for HMGB1 antibody 10829-1-AP||Download protocol|
|IHC protocol for HMGB1 antibody 10829-1-AP||Download protocol|
|IF protocol for HMGB1 antibody 10829-1-AP||Download protocol|
|IP protocol for HMGB1 antibody 10829-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Hydrogen gas reduces HMGB1 release in lung tissues of septic mice in an Nrf2/HO-1-dependent pathway.
Cardiovasc Drugs Ther
Endogenous Neurosteroid (3α,5α)3-Hydroxypregnan-20-one Inhibits Toll-like-4 Receptor Activation and Pro-inflammatory Signaling in Macrophages and Brain.
Am J Physiol Heart Circ Physiol
NRP1 regulates HMGB1 in vascular endothelial cells under high homocysteine condition.
Rosmarinic acid attenuates inflammatory responses through inhibiting HMGB1/TLR4/NF-κB signaling pathway in a mouse model of Parkinson's disease.
Int J Biol Macromol
Modulating the gut microbiota and inflammation is involved in the effect of Bupleurum polysaccharides against diabetic nephropathy in mice.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
PATRICK (Verified Customer) (12-01-2018)
This antibody worked well. ProteinTech provides antibody specific instructions that were helpful for each of the antibodies we ordered.