Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, mouse spleen tissue, rat spleen tissue, L02 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue |
| Positive IF-Fro detected in | mouse spleen tissue |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10701-1-AP targets HO-1/HMOX1 in WB, IHC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, canine, monkey, chicken, hamster, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1190 Product name: Recombinant human HO-1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-288 aa of BC001491 Sequence: MERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEVIPYTPAMQHYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAFLLNIQLFEELQELLTHDTKDQSPSRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQAPLLRWVLTLSFLVATVAVGLYAM Predict reactive species |
| Full Name | heme oxygenase (decycling) 1 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 28-33 kDa |
| GenBank Accession Number | BC001491 |
| Gene Symbol | HO-1 |
| Gene ID (NCBI) | 3162 |
| RRID | AB_2118685 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P09601 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for HO-1/HMOX1 antibody 10701-1-AP | Download protocol |
| IF protocol for HO-1/HMOX1 antibody 10701-1-AP | Download protocol |
| IHC protocol for HO-1/HMOX1 antibody 10701-1-AP | Download protocol |
| IP protocol for HO-1/HMOX1 antibody 10701-1-AP | Download protocol |
| WB protocol for HO-1/HMOX1 antibody 10701-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HO-1/HMOX1 polyclonal antibody (Cat# 10701-1-AP) has accumulated 1,910 citations across high-impact journals including Nature, Cell Metabolism, Nature Communications, Nature Cell Biology, Science Translational Medicine, Redox Biology, Journal of Advanced Research, Advanced Science, Cell Death & Disease, and Phytomedicine. Citations span research fields such as ferroptosis, oxidative stress and redox biology, Nrf2/HO-1 signaling, oncology, neuroprotection, cardiovascular disease, liver and kidney injury, inflammation, nanomedicine, and metabolic disorders.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther FGF1ΔHBS prevents diabetic cardiomyopathy by maintaining mitochondrial homeostasis and reducing oxidative stress via AMPK/Nur77 suppression. | ||
Signal Transduct Target Ther Acevaltrate as a novel ferroptosis inducer with dual targets of PCBP1/2 and GPX4 in colorectal cancer | ||
Circulation ALDH2/eIF3E Interaction Modulates Protein Translation Critical for Cardiomyocyte Ferroptosis in Acute Myocardial Ischemia Injury | ||
Cell Metab Functional Genomics In Vivo Reveal Metabolic Dependencies of Pancreatic Cancer Cells. | ||
Cell Metab Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals |
Reviews
What customers say
All six reviewers rate this HO-1 (HMOX1) antibody highly (5/5 stars, one 4/5). It performs reliably in Western blot and immunofluorescence across mouse heart tissue, spleen, A549, HepG2 cells, and LPS-stimulated macrophages. Users report a clean band at 28–33 kDa with dilutions ranging from 1:1000 to 1:3000. Several highlight its extensive peer-reviewed citations (~1300+) as a mark of confidence. Overall, reviewers describe it as a strong, well-supported choice for studying HO-1 expression and regulation.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sai Sindhura (Verified Customer) (08-19-2026) | HO-1 / HMOX very good antibody
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Leila (Verified Customer) (02-23-2026) | The 10701-1-AP antibody is a reliable and well-supported antibody for researchers studying HO-1 expression and regulation. Its extensive literature backing and broad application support make it a strong option for most standard experimental needs in cellular and molecular biology. This antibody is very well cited in peer-reviewed research (~1300+ citations), which gives confidence that many labs have successfully used it for diverse applications.
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Brice-Emmanuel (Verified Customer) (12-18-2023) | Works perfectly. Band a 28-33 kDa
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James (Verified Customer) (11-18-2022) | Very good antibody! Used it for IF staining of paraffin-embedded spleen tissue. HMOX-1 positive cells are in Green and CD45 in red.
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Hala (Verified Customer) (07-28-2021) | incubation 1.5 hours at room temperature dilution 1/3000
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JIM (Verified Customer) (03-26-2019) | This antibody offers a good detection of HO-1 expression in LPS stimulated macrophages. The HO-1 expression can be observed in the cells after 24h stimulation of LPS.
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