HO-1/HMOX1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HO-1/HMOX1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

487

Reviews (2)

Cat no : 10701-1-AP

Synonyms

bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HO-1, HO-1/HMOX1, HSP32

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Validation Data Gallery

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  WB analysis of HeLa using 10701-1-AP

  WB result of HMOX1 antibody (10701-1-AP, 1:3000) with si-Control and si-HMOX1 transfected HeLa cells.

• 

  WB analysis using 10701-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10701-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HMOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10701-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10701-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10701-1-AP

  IP Result of anti-HMOX1 (IP:10701-1-AP, 3ug; Detection:10701-1-AP 1:1000) with HeLa cells lysate 3000ug.

• 

  IHC staining of human liver cancer using 10701-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10701-1-AP (HMOX1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10701-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10701-1-AP (HMOX1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10701-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10701-1-AP (HMOX1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 10701-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10701-1-AP (HMOX1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human liver cancer using 10701-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using HO-1/HMOX1 antibody (10701-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 10701-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HO-1/HMOX1 (10701-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HT-1080 cells, HeLa cells, rat spleen tissue, HepG2 cells, mouse liver tissue, rat liver tissue, mouse kidney tissue, mouse spleen tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human liver cancer tissue
• Positive FC detected in: HeLa cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 11 publications below
• WB: See 457 publications below
• IHC: See 37 publications below
• IF: See 30 publications below
• IP: See 2 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below

Product Information

10701-1-AP targets HO-1/HMOX1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, Bovine, chicken, monkey, pig, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HO-1/HMOX1 fusion protein Ag1190
• Full Name: heme oxygenase (decycling) 1
• Calculated molecular weight: 33 kDa
• Observed molecular weight: 28-33 kDa
• GenBank accession number: BC001491
• Gene symbol: HMOX1
• Gene ID (NCBI): 3162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Publications

Species	Application	Title
human	WB,IF,IP	J Exp Clin Cancer Res; 14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.; Authors - Jia Song ; KD Validated
mouse	WB	Int Immunopharmacol; Hepatoprotective effects and structure-activity relationship of five flavonoids against lipopolysaccharide/d-galactosamine induced acute liver failure in mice.; Authors - Yutong He
human	IF	Cancer Sci; Nuclear factor (erythroid-derived 2)-like 2 antioxidative response mitigates cytoplasmic radiation-induced DNA double-strand breaks.; Authors - Jun Wang
mouse	WB	Sci Rep; The harmful effects of acute PM2.5 exposure to the heart and a novel preventive and therapeutic function of CEOs.; Authors - Lu Dong
mouse	WB	ACS Chem Neurosci; Neuroprotective Effect of Swertiamain on Cerebral Ischemia/Reperfusion Injury by Inducing the Nrf2 Protective Pathway.; Authors - Hui Wang
rat	WB	Biol Pharm Bull; Astragaloside IV regulates the PI3K/Akt/HO-1 signaling pathway and inhibits H9c2 cardiomyocyte injury induced by hypoxia-reoxygenation.; Authors - Ping Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hala (Verified Customer) (07-28-2021)

incubation 1.5 hours at room temperature dilution 1/3000

• Applications: Western Blot
• Primary Antibody Dilution: 1/3000
• Cell Tissue Type: HepG2

HO-1/HMOX1 Antibody Western Blot validation (1/3000 dilution) in HepG2 (Cat no:10701-1-AP)

FH

JIM (Verified Customer) (03-26-2019)

This antibody offers a good detection of HO-1 expression in LPS stimulated macrophages. The HO-1 expression can be observed in the cells after 24h stimulation of LPS.

• Applications: Western Blot,

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