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HNRNPC Polyclonal antibody

HNRNPC Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 11760-1-AP

Synonyms

C1, C2, HNRNP, hnRNP C1/C2, HNRNPC, HNRPC, SNRPC



Tested Applications

Positive WB detected inHeLa cells, human brain tissue, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11760-1-AP targets HNRNPC in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPC fusion protein Ag2356
Full Name heterogeneous nuclear ribonucleoprotein C (C1/C2)
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa, 36-40 kDa
GenBank accession numberBC003394
Gene symbol HNRNPC
Gene ID (NCBI) 3183
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HNRNPC, also named as Heterogeneous nuclear ribonucleoproteins C1/C2, is a 306 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and belongs to the RRM HNRPC family. HNRNPC localizes in the nucleus. It binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. HNRNPC may play a role in the early steps of spliceosome assembly and pre-mRNA splicing. HNRNPC can act as a tetramer and is involved in the assembly of 40S hnRNP particles and plays a role with CSDE1 in internal initiation of translation during mitosis.

Protocols

Product Specific Protocols
WB protocol for HNRNPC antibody 11760-1-APDownload protocol
IHC protocol for HNRNPC antibody 11760-1-APDownload protocol
IF protocol for HNRNPC antibody 11760-1-APDownload protocol
IP protocol for HNRNPC antibody 11760-1-APDownload protocol
FC protocol for HNRNPC antibody 11760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Med Sci Monit

Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.

Authors - Yingying Zhang
mouseWB

Neurosci Lett

hnRNPM deficiency leads to cognitive deficits via disrupting synaptic plasticity.

Authors - Akinsola Raphael Akinyemi
humanWB

Mar Drugs

An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells.

Authors - Su Ching-Chyuan CC
humanWB,IHC

Front Oncol

M6A RNA Methylation Regulator HNRNPC Contributes to Tumorigenesis and Predicts Prognosis in Glioblastoma Multiforme.

Authors - Li-Chong Wang
humanWB

Nucleic Acids Res

Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.

Authors - Misbah Khan
mouseWB

Nature

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Authors - Wenqi Xu