|Positive WB detected in||HeLa cells, human brain tissue, K-562 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11760-1-AP targets HNRNPC in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HNRNPC fusion protein Ag2356|
|Full Name||heterogeneous nuclear ribonucleoprotein C (C1/C2)|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||32 kDa, 36-40 kDa|
|GenBank accession number||BC003394|
|Gene ID (NCBI)||3183|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HNRNPC, also named as Heterogeneous nuclear ribonucleoproteins C1/C2, is a 306 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and belongs to the RRM HNRPC family. HNRNPC localizes in the nucleus. It binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. HNRNPC may play a role in the early steps of spliceosome assembly and pre-mRNA splicing. HNRNPC can act as a tetramer and is involved in the assembly of 40S hnRNP particles and plays a role with CSDE1 in internal initiation of translation during mitosis.
|Product Specific Protocols|
|WB protocol for HNRNPC antibody 11760-1-AP||Download protocol|
|IHC protocol for HNRNPC antibody 11760-1-AP||Download protocol|
|IF protocol for HNRNPC antibody 11760-1-AP||Download protocol|
|IP protocol for HNRNPC antibody 11760-1-AP||Download protocol|
|FC protocol for HNRNPC antibody 11760-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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