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HNRNPC Polyclonal antibody

HNRNPC Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 11760-1-AP

Synonyms

C1, C2, HNRNP, hnRNP C1/C2, HNRNPC, HNRPC, SNRPC



Tested Applications

Positive WB detected inHeLa cells, human brain tissue, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11760-1-AP targets HNRNPC in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPC fusion protein Ag2356
Full Name heterogeneous nuclear ribonucleoprotein C (C1/C2)
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC003394
Gene symbol HNRNPC
Gene ID (NCBI) 3183
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HNRNPC, also named as Heterogeneous nuclear ribonucleoproteins C1/C2, is a 306 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and belongs to the RRM HNRPC family. HNRNPC localizes in the nucleus. It binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. HNRNPC may play a role in the early steps of spliceosome assembly and pre-mRNA splicing. HNRNPC can act as a tetramer and is involved in the assembly of 40S hnRNP particles and plays a role with CSDE1 in internal initiation of translation during mitosis.

Protocols

Product Specific Protocols
WB protocol for HNRNPC antibody 11760-1-APDownload protocol
IHC protocol for HNRNPC antibody 11760-1-APDownload protocol
IF protocol for HNRNPC antibody 11760-1-APDownload protocol
IP protocol for HNRNPC antibody 11760-1-APDownload protocol
FC protocol for HNRNPC antibody 11760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Med Sci Monit

Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.

Authors - Yingying Zhang
mouseWB

Neurosci Lett

hnRNPM deficiency leads to cognitive deficits via disrupting synaptic plasticity.

Authors - Akinsola Raphael Akinyemi
humanWB

Mar Drugs

An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells.

Authors - Su Ching-Chyuan CC
humanWB,IHC

Front Oncol

M6A RNA Methylation Regulator HNRNPC Contributes to Tumorigenesis and Predicts Prognosis in Glioblastoma Multiforme.

Authors - Li-Chong Wang
mouseWB

Nature

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Authors - Wenqi Xu