HOXA5 Polyclonal antibody

• 

  WB analysis using 27622-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27622-1-AP (HOXA5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 27622-1-AP

  IP result of anti-HOXA5 (IP:27622-1-AP, 4ug; Detection:27622-1-AP 1:500) with HEK-293 cells lysate 1360 ug.

HOXA5 Polyclonal Antibody for WB, IP, ELISA

Cat No. 27622-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

homeobox A5, Homeobox protein Hox 1C, Homeobox protein Hox A5, Homeobox protein Hox-1C, Homeobox protein Hox-A5

---

Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27622-1-AP targets HOXA5 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HOXA5 fusion protein Ag26362
• Full Name: homeobox A5
• Calculated Molecular Weight: 270 aa, 29 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC013682
• Gene Symbol: HOXA5
• Gene ID (NCBI): 3202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20719
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HOXA5 (Homeobox A5) is a member of the homeobox gene family, which plays a crucial role in embryonic development, cell differentiation, and tissue patterning (PMID: 31159758). These genes encode transcription factors containing a conserved homeodomain that binds DNA to regulate the expression of target genes involved in morphogenesis and organogenesis.