HP1BP3 Recombinant monoclonal antibody

• 

  WB analysis using 86974-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86974-1-RR (HP1BP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

HP1BP3 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86974-1-RR

Clone No.252095F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Heterochromatin protein 1-binding protein 3, HP1 BP74, Protein HP1 BP74, Protein HP1-BP74

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86974-1-RR targets HP1BP3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag18709

  Product name: Recombinant human HP1BP3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-302 aa of BC045660

  Sequence: MASSPRPKMDAILTEAIKACFQKSGASVVAIRKYIIHKYPSLELERRGYLLKQALKRELNRGVIKQVKGKGASGSFVVVQKSRKTPQKSRNRKNRSSAVDPEPQVKLEDVLPLAFTRLCEPKEASYSLIRKYVSQYYPKLRVDIRPQLLKNALQRAVERGQLEQITGKGASGTFQLKKSGEKPLLGGSLMEYAILSAIAAMNEPKTCSTTALKKYVLENHPGTNSNYQMHLLKKTLQKCEKNGWMEQISGKGFSGTFQLCFPYYPSPGVLFPKKEPDDSRDEDEDEDESSEEDSEDEEPPPK

• Full Name: heterochromatin protein 1, binding protein 3
• Calculated Molecular Weight: 553 aa, 61 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC045660
• Gene Symbol: HP1BP3
• Gene ID (NCBI): 50809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5SSJ5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Heterochromatin protein 1 binding protein 3(HP1BP3) associates with chromatin throughout the cell cycle and is required to maintain higher order chromatin structure during metaphase. HP1BP3 as a novel modulator of cognitive aging using a genetically diverse population of mice and confirm that HP1BP3 protein levels are significantly reduced in the hippocampi of cognitively impaired elderly humans relative to cognitively intact controls. Double-band staining is typical of HP1BP3 expression, indicative of multiple splice variants present in brain tissue.