HPF1 Polyclonal antibody

HPF1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28456-1-AP

Synonyms

C4orf27, FLJ20534, FLJ33423, FLJ42042, HPF1, UPF0609 protein C4orf27

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Validation Data Gallery

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  WB analysis using 28456-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28456-1-AP (HPF1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, MOLT-4 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28456-1-AP targets HPF1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HPF1 fusion protein Ag29377
• Full Name: chromosome 4 open reading frame 27
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC010367
• Gene Symbol: C4orf27
• Gene ID (NCBI): 54969
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HPF1 is also known as C4orf27 and belongs to the HPF1 family. HPF1 is a novel interacting component of PARP1 complex that plays an important role in PARP1-dependent ADP-ribosylation signaling in the DNA damage response and the maintenance of genome stability (PMID: 29549427). The calculated molecular weight of HPF1 is 39 kDa.