HTR5A Polyclonal antibody

HTR5A Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

IHC,ELISA

Conjugate

Unconjugated

Cat no : 28221-1-AP

Synonyms

5 HT 5, 5 HT 5A, 5 HT5A, HTR5A, Serotonin receptor 5A

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Validation Data Gallery

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  IHC staining of mouse brain using 28221-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28221-1-AP (HTR5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28221-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28221-1-AP (HTR5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28221-1-AP targets HTR5A in IHC,ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HTR5A fusion protein Ag28177
• Full Name: 5-hydroxytryptamine (serotonin) receptor 5A
• Calculated Molecular Weight: 357 aa, 48 kDa
• GenBank Accession Number: BC112021
• Gene Symbol: HTR5A
• Gene ID (NCBI): 3361
• RRID: AB_2918151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.