Histone H3 Monoclonal antibody

Histone H3 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b


Human, mouse, rat, chicken, zebrafish, wheat







Cat no : 68345-1-Ig


H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone cluster 1, H3a, Histone H3, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, Histone-H3

Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, chicken brain tissue, zebrafish, wheat whole plant
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, A431 cells, HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

68345-1-Ig targets Histone H3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, chicken, zebrafish, wheat samples.

Tested Reactivity Human, mouse, rat, chicken, zebrafish, wheat
Cited Reactivitymouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name histone cluster 1, H3a
Observed molecular weight15 kDa
GenBank accession numberBC066245
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.


Product Specific Protocols
WB protocol for Histone H3 antibody 68345-1-IgDownload protocol
IHC protocol for Histone H3 antibody 68345-1-IgDownload protocol
IF protocol for Histone H3 antibody 68345-1-IgDownload protocol
IP protocol for Histone H3 antibody 68345-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Redox Biol

Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice

Authors - Boying Zhao