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Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, MCF-7 cells, HEK-293 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66597-1-Ig targets CHCHD6 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14700 Product name: Recombinant human CHCHD6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-235 aa of BC006123 Sequence: MGSTESSEGRRVSFGVDEEERVRVLQGVRLSENVVNRMKEPSSPPPAPTSSTFGLQDGNLRAPHKESTLPRSGSSGGQQPSGMKEGVKRYEQEHAAIQDKLFQVAKREREAATKHSKASLPTGEGSISHEEQKSVRLARELESREAELRRRDTFYKEQLERIERKNAEMYKLSSEQFHEAASKMESTIKPRRVEPVCSGLQAQILHCYRDRPHEVLLCSDLVKAYQRCVSAAHKG Predict reactive species |
| Full Name | coiled-coil-helix-coiled-coil-helix domain containing 6 |
| Calculated Molecular Weight | 235 aa, 26 kDa |
| Observed Molecular Weight | 26-29 kDa |
| GenBank Accession Number | BC006123 |
| Gene Symbol | CHCHD6 |
| Gene ID (NCBI) | 84303 |
| RRID | AB_2881957 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BRQ6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CHCHD6 antibody 66597-1-Ig | Download protocol |
| WB protocol for CHCHD6 antibody 66597-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
