Validation Data Gallery
|Positive WB detected in
|LNCaP cells, HeLa cells, Recombinant protein, HEK-293 cells, Jurkat cells, ROS1728 cells, C6 cells, RAW 264.7 cells, NIH/3T3 cells, HepG2 cells, K-562 cells, HSC-T6 cells, 4T1 cells
|Positive IHC detected in
|human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:20000-1:100000
|IHC : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66442-1-Ig targets Hsc70 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, goat, pig
|Host / Isotype
|Mouse / IgG1
|Hsc70 fusion protein Ag17910
|heat shock 70kDa protein 8
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease. (PMID: 21832061)
p38-TFEB pathways promote microglia activation through inhibiting CMA-mediated NLRP3 degradation in Parkinson's disease.
Heat Shock Protein Member 8 (HSPA8) Is Involved in Porcine Reproductive and Respiratory Syndrome Virus Attachment and Internalization.
Cell Commun Signal
Discovery of LAMP-2A as potential biomarkers for glioblastoma development by modulating apoptosis through N-CoR degradation.
FEBS Open Bio
A curcumin analogue GO-Y030 depletes cancer stem cells by inhibiting the interaction between the HSP70/HSP40 complex and its substrates
Ecotoxicol Environ Saf
PARP1 promotes NLRP3 activation via blocking TFEB-mediated autophagy in rotenone-induced neurodegeneration
J Transl Med
Arginine methylation of HSPA8 by PRMT9 inhibits ferroptosis to accelerate hepatitis B virus-associated hepatocellular carcinoma progression