Hsc70 Monoclonal antibody

Hsc70 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat and More (1)







Cat no : 66442-1-Ig


Heat shock 70 kDa protein 8, Hsc70, HSC71, HSP71, HSP73, HSPA10, HSPA8

Tested Applications

Positive WB detected inHeLa cells, Recombinant protein protein
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:3000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66442-1-Ig targets Hsc70 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Hsc70 fusion protein Ag17910
Full Name heat shock 70kDa protein 8
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC007276
Gene symbol HSPA8
Gene ID (NCBI) 3312
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease. (PMID: 21832061)


Product Specific Protocols
WB protocol for Hsc70 antibody 66442-1-IgDownload protocol
IHC protocol for Hsc70 antibody 66442-1-IgDownload protocol
IF protocol for Hsc70 antibody 66442-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Commun Signal

Discovery of LAMP-2A as potential biomarkers for glioblastoma development by modulating apoptosis through N-CoR degradation.

Authors - Yongjie Wang

Microbiol Spectr

Heat Shock Protein Member 8 (HSPA8) Is Involved in Porcine Reproductive and Respiratory Syndrome Virus Attachment and Internalization.

Authors - Lei Wang

J Neuroinflammation

p38-TFEB pathways promote microglia activation through inhibiting CMA-mediated NLRP3 degradation in Parkinson's disease.

Authors - Jialong Chen