Hsc70 Monoclonal antibody

Hsc70 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A4B3

Cat no : 66442-1-Ig

Synonyms

Heat shock 70 kDa protein 8, Hsc70, HSC71, HSP71, HSP73, HSPA10, HSPA8



Tested Applications

Positive WB detected inLNCaP cells, Recombinant protein, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, ROS1728 cells, C6 cells, RAW 264.7 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66442-1-Ig targets Hsc70 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, goat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Hsc70 fusion protein Ag17910
Full Name heat shock 70kDa protein 8
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC007276
Gene symbol HSPA8
Gene ID (NCBI) 3312
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease. (PMID: 21832061)

Protocols

Product Specific Protocols
WB protocol for Hsc70 antibody 66442-1-IgDownload protocol
IHC protocol for Hsc70 antibody 66442-1-IgDownload protocol
IF protocol for Hsc70 antibody 66442-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neuroinflammation

p38-TFEB pathways promote microglia activation through inhibiting CMA-mediated NLRP3 degradation in Parkinson's disease.

Authors - Jialong Chen
human,pigWB,IF

Microbiol Spectr

Heat Shock Protein Member 8 (HSPA8) Is Involved in Porcine Reproductive and Respiratory Syndrome Virus Attachment and Internalization.

Authors - Lei Wang
humanWB

Cell Commun Signal

Discovery of LAMP-2A as potential biomarkers for glioblastoma development by modulating apoptosis through N-CoR degradation.

Authors - Yongjie Wang
humanWB

FEBS Open Bio

A curcumin analogue GO-Y030 depletes cancer stem cells by inhibiting the interaction between the HSP70/HSP40 complex and its substrates

Authors - Maya Suzuki

Ecotoxicol Environ Saf

PARP1 promotes NLRP3 activation via blocking TFEB-mediated autophagy in rotenone-induced neurodegeneration

Authors - He Zhang
goatWB

Int J Mol Sci

Integrative Proteomics and Transcriptomics Profiles of the Oviduct Reveal the Prolificacy-Related Candidate Biomarkers of Goats (Capra hircus) in Estrous Periods

Authors - Zhipeng Sun