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Hsc70 Monoclonal antibody

Hsc70 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A4B3

Cat no : 66442-1-Ig

Synonyms

Heat shock 70 kDa protein 8, Heat shock cognate 71 kDa protein, Hsc70, HSC71, HSP71, HSP73, HSP7C, HSPA10, HSPA8



Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, Recombinant protein, HEK-293 cells, Jurkat cells, ROS1728 cells, C6 cells, RAW 264.7 cells, NIH/3T3 cells, HepG2 cells, K-562 cells, HSC-T6 cells, 4T1 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66442-1-Ig targets Hsc70 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, goat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Hsc70 fusion protein Ag17910
Full Name heat shock 70kDa protein 8
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC007276
Gene symbol HSPA8
Gene ID (NCBI) 3312
RRIDAB_2881812
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease. (PMID: 21832061)

Protocols

Product Specific Protocols
WB protocol for Hsc70 antibody 66442-1-IgDownload protocol
IHC protocol for Hsc70 antibody 66442-1-IgDownload protocol
IF protocol for Hsc70 antibody 66442-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neuroinflammation

p38-TFEB pathways promote microglia activation through inhibiting CMA-mediated NLRP3 degradation in Parkinson's disease.

Authors - Jialong Chen
human,pigWB,IF

Microbiol Spectr

Heat Shock Protein Member 8 (HSPA8) Is Involved in Porcine Reproductive and Respiratory Syndrome Virus Attachment and Internalization.

Authors - Lei Wang
humanWB

Cell Commun Signal

Discovery of LAMP-2A as potential biomarkers for glioblastoma development by modulating apoptosis through N-CoR degradation.

Authors - Yongjie Wang
humanWB

FEBS Open Bio

A curcumin analogue GO-Y030 depletes cancer stem cells by inhibiting the interaction between the HSP70/HSP40 complex and its substrates

Authors - Maya Suzuki

Ecotoxicol Environ Saf

PARP1 promotes NLRP3 activation via blocking TFEB-mediated autophagy in rotenone-induced neurodegeneration

Authors - He Zhang
humanIF

J Transl Med

Arginine methylation of HSPA8 by PRMT9 inhibits ferroptosis to accelerate hepatitis B virus-associated hepatocellular carcinoma progression

Authors - Wensheng Deng