CoraLite® Plus 488-conjugated Hsc70 Monoclonal antibody

Hsc70 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A4B3

Cat no : CL488-66442

Synonyms

Heat shock 70 kDa protein 8, heat shock 70kDa protein 8, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, HSPA8, LAP1, NIP71

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Validation Data Gallery

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  IF Staining of HEK-293 using CL488-66442

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 Hsc70 antibody (CL488-66442, Clone: 1A4B3 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66442 targets Hsc70 in IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Hsc70 fusion protein Ag17910
• Full Name: heat shock 70kDa protein 8
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC007276
• Gene Symbol: HSPA8
• Gene ID (NCBI): 3312
• RRID: AB_2919326
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease. (PMID: 21832061)