Human ACE ELISA Kit
Cat no : KE00762
Synonyms
ACE1, Angiotensin converting enzyme, Angiotensin-converting enzyme, Angiotensin-converting enzyme, soluble form, DCP
Validation Data Gallery
Product Information
KE00762 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ACE ELISA kit is to be used to detect and quantify protein levels of endogenous human ACE. The assay recognizes human ACE. An antibody specific for human ACE has been pre-coated onto the microwells. The human ACE protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ACE is added to detect the captured human ACE protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ACE ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture superntant, Saliva |
| Assay type | Sandwich |
| Sensitivity | 0.72 ng/mL |
| Range | 1.25-80 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1636 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatant | 118% | 113%-122% |
| Saliva | 120% | 113%-129% |
| Human serum | 107% | 105%-108% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 37.64 | 1.74 | 4.62 |
| 2 | 8 | 13.28 | 0.55 | 4.17 |
| 3 | 8 | 7.62 | 0.42 | 5.51 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 34.73 | 3.59 | 10.33 |
| 2 | 16 | 12.64 | 0.81 | 6.44 |
| 3 | 16 | 7.54 | 0.37 | 4.90 |
Background Information
Angiotensin-converting enzyme (ACE) was initially discovered in 1953 during the study of the renin-angiotensin system (RAS). ACE plays a part in blood pressure regulation by converting angiotensin I to angiotensin II. ACE is upregulated in several diseases characterized by granulomas, including active sarcoidosis, histoplasmosis, leprosy, silicosis, granulomatosis with polyangiitis and the granulomatous reaction induced by murine schistosomiasis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ACE1, Angiotensin converting enzyme, Angiotensin-converting enzyme, Angiotensin-converting enzyme, soluble form, DCP |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.