Human ACE2 ELISA Kit
Cat no : KE00254
Synonyms
ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15
Validation Data Gallery
Product Information
KE00254 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Human ACE2 ELISA kit is to be used to detect and quantify protein levels of endogenous human ACE2. The assay recognizes human ACE2. An antibody specific for human ACE2 has been pre-coated onto the microwells. The human ACE2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody for human ACE2 is added to detect the captured human ACE2 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ACE2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.15 pg/mL |
| Range | 6.25 - 400 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 59272 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 108.1% | 90%-124% |
| Urine | 85.0% | 75%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 181.9 | 6.0 | 3.3 |
| 2 | 20 | 46.9 | 1.5 | 3.1 |
| 3 | 20 | 11.4 | 0.7 | 6.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 191.7 | 12.0 | 6.3 |
| 2 | 24 | 47.6 | 2.5 | 5.2 |
| 3 | 24 | 12.3 | 1.0 | 8.2 |
Background Information
Angiotensin-converting enzyme 2 (ACE2), a critical regulator of the renin-angiotensin system (RAS), belongs to the peptidase M2 family. It plays an important role in cardiovascular homeostasis by regulating vascular tone, fluid, and electrolyte balance. ACE2 functions as a carboxymonopeptidase hydrolyzing the cleavage of a single C-terminal residue from Angiotensin-II (Ang-II), the key peptide hormone of RAS, to form Angiotensin-(1-7) (Ang-(1-7)), which binds to the G-protein-coupled Mas receptor and activates signaling pathways that counteract the pathways activated by Ang-II. ACE2 is expressed in a variety of tissues, including the kidneys, testes, heart, and intestines, and is particularly enriched in lung epithelium. Recent studies have shown that increased ACE2 expression is likely to help prevent secondary fibrosis changes following COVID-19 pneumonia.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
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