Recombinant Human ALPI protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3947

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Validation Data Gallery

• 

  Purity of Recombinant Human ALPI was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human ALPI protein Val20-Asp503 (Accession# P09923) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 248
• Accession: P09923
• PredictedSize: 78.7 kDa
• SDS-PAGE: 83-100 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

There are at least four alkaline phosphatase isozymes (ALPs), i.e., tissue-unspecific (liver/bone/kidney), intestinal (I-), placental (P-) and germ cell or placental-like (P-like). IAP (Intestinal-type alkaline phosphatase), also known as ALPI, is found exclusively in tubuloepithelial cells of the S3-segment of the proximal tubule. IAP is mainly distributed in the intestines, but exigous synthesis has been observed in kidney, liver, and lung tissue. The sucrase and IAP are useful functional markers for intestinal epithelial cell-type cancer cells.

References:

1. Verpooten, G. F., et al. (1989). Kidney Int. Oct;36(4):617-25. 
2. Giatromanolaki A, et al. (2002). Semin Oncol. Dec;29(6 Suppl 19):14-21. 
3. Nakamura W, et al. (1998). Jpn J Cancer Res. Feb;89(2):186-91. 
4. Harada T, et al. (2003). Am J Physiol Gastrointest Liver Physiol. Feb;284(2):G255-G262.