Speedy™ Human APEX1 One-Step ELISA Kit
Cat no : SE50283
Synonyms
APE, APE1, APE-1, APEN, APEX
Validation Data Gallery
Product Information
| Product name | Speedy™ Human APEX1 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 328 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 89% | 82%-103% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 21.66 | 0.72 | 3.32 |
| 2 | 8 | 5.08 | 0.14 | 2.76 |
| 3 | 8 | 2.53 | 0.11 | 4.35 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 22.24 | 1.24 | 5.58 |
| 2 | 16 | 5.16 | 0.22 | 4.26 |
| 3 | 16 | 2.55 | 0.11 | 4.31 |
Background Information
APEX1 (Apurinic/Apyrimidinic Endonuclease 1), also known as APE1, HAP1, or Ref-1, is a multifunctional DNA repair enzyme. This protein is renowned for its bifunctional properties: on one hand, APEX1 is a core component of the base excision repair (BER) pathway, initiating DNA damage repair by cleaving apurinic/apyrimidinic (AP) sites to maintain genomic stability. On the other hand, APEX1 possesses redox activity, capable of reductively activating various transcription factors (such as AP-1, NF-κB, p53, HIF-1α, etc.) to regulate gene expression. Furthermore, this protein is involved in ribonucleotide reductase regulation, RNA processing, and mitochondrial DNA repair. APEX1 is highly expressed in various tumors and is closely associated with tumor drug resistance, radioresistance, and poor prognosis, making it a potential therapeutic target and prognostic marker. Its functional deficiency is linked to neurodegenerative diseases, cancer susceptibility, and aging.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | APE, APE1, APE-1, APEN, APEX |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.