Human APOB-100 ELISA Kit
Cat no : KE00158
Synonyms
APOB, Apolipoprotein B, Apo B, Apo B-100, ApoB100
Validation Data Gallery
Product Information
KE00158 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The APOB ELISA kit is to be used to detect and quantify protein levels of endogenous APOB. The assay recognizes human APOB. An antibody specific for APOB has been pre-coated onto the microwells. The APOB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human APOB is added to detect the captured human APOB protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human APOB-100 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma, Cell Culture supernatants, Human milk |
| Assay type | Sandwich |
| Sensitivity | 0.68 ng/mL |
| Range | 3.9-250 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 338 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Serum | 95% | 77%-121% |
| Cell Culture supernatants | 103% | 86%-122% |
| Human milk | 102% | 75%-122% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 119.9 | 4.1 | 3.4 |
| 2 | 20 | 28.3 | 0.7 | 2.6 |
| 3 | 20 | 7.2 | 0.4 | 5.4 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 133.3 | 9.8 | 7.3 |
| 2 | 24 | 31.0 | 2.4 | 7.9 |
| 3 | 24 | 8.0 | 0.8 | 10.0 |
Background Information
The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB gene encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-100 is synthesized exclusively by the liver and is essential for the assembly of VLDL in the liver. APOB-48 is synthesized exclusively by the small intestine and is essential for the assembly of chylomicrons in the intestine. APOB-48 and APOB-100 share a common N-terminal sequence, but APOB-48 lacks APOB-100's C-terminal LDL receptor binding region. Plasma APOB levels are associated with coronary heart disease. The Kit is specific for APOB-100 and does not recognize APOB-48.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | APOB, Apolipoprotein B, Apo B, Apo B-100, ApoB100 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
J Nat Med Inhibitory effect of trans-tiliroside on very low-density lipoprotein secretion in HepG2 cells and mouse liver | ||
J Gen Virol Hepatitis B virus is a stealth virus that minimizes proteomic and secretomic changes in primary human hepatocytes. |