Human APOL1 ELISA Kit
Cat no : KE00047
Synonyms
APOL, APOL1, Apolipoprotein L, Apolipoprotein L1
Validation Data Gallery
Product Information
KE00047 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The APOL1 ELISA kit is to be used to detect and quantify protein levels of endogenous APOL1. The assay recognizes human APOL1. An antibody specific for APOL1 has been pre-coated onto the microwells. The APOL1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for APOL1 is added to detect the captured APOL1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human APOL1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.07 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8542 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 105% | 83%-127% |
| Human plasma | 93% | 85%-107% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 8.31 | 0.312 | 3.8 |
| 2 | 20 | 2.21 | 0.095 | 4.3 |
| 3 | 20 | 0.63 | 0.013 | 2.1 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 9.12 | 0.387 | 4.2 |
| 2 | 24 | 2.31 | 0.073 | 3.2 |
| 3 | 24 | 0.62 | 0.023 | 3.6 |
Background Information
Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles. The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. This kit is used to quantify APOL1 level.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | APOL, APOL1, Apolipoprotein L, Apolipoprotein L1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Nat Med A tripartite complex of suPAR, APOL1 risk variants and αvβ3 integrin on podocytes mediates chronic kidney disease. | ||
Immunity APOL1 risk variants in individuals of African genetic ancestry drive endothelial cell defects that exacerbate sepsis. | ||
Alzheimers Dement (Amst) Novel diagnostic cerebrospinal fluid biomarkers for pathologic subtypes of frontotemporal dementia identified by proteomics. | ||
Sci Rep Circulating Apolipoprotein L1 is associated with insulin resistance-induced abnormal lipid metabolism. | ||
Exp Mol Pathol Vascular smooth muscle cells contribute to APOL1-induced podocyte injury in HIV milieu. | ||
JCI Insight Antisense oligonucleotide treatment ameliorates IFN-γ-induced proteinuria in APOL1-transgenic mice. |