Human Adiponectin ELISA Kit
Cat no : KE00290
Synonyms
Adiponectin,ADIPOQ,30 kDa adipocyte complement-rel
Validation Data Gallery
Product Information
KE00290 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Adiponectin ELISA kit is to be used to detect and quantify protein levels of endogenous Adiponectin. The assay recognizes human Adiponectin. An antibody specific for Adiponectin has been pre-coated onto the microwells. The Adiponectin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for Adiponectin is added to detect the captured Adiponectin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Adiponectin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant，Cell Lysate |
| Assay type | Sandwich |
| Sensitivity | 1.9 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9370 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 105% | 94%-129% |
| Cell culture supernatant | 115% | 107%-130% |
| Cell Lysate | 89% | 71%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,942.2 | 78.6 | 4.0 |
| 2 | 20 | 468.8 | 20.8 | 4.4 |
| 3 | 20 | 126.4 | 6.5 | 5.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,981.1 | 131.0 | 6.6 |
| 2 | 24 | 477.8 | 23.5 | 4.9 |
| 3 | 24 | 125.9 | 8.8 | 7.0 |
Background Information
Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Human adiponectin exists in three multimer forms, high-molecular-weight (HMW), middle-molecular-weight (MMW), and low-molecular-weight (LMW). Adiponectin modulates a number of metabolic processes, including improving insulin sensitivity and anti-inflammatory activity. The role of Adiponectin in reproduction is not yet fully understood, but the expression of Adiponectin in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane Adiponectin receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. Adiponectin May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Adiponectin,ADIPOQ,30 kDa adipocyte complement-rel |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Sci Rep Large-scale proteomic analysis of aqueous humor from patients with primary open angle and exfoliation glaucoma. |