Speedy™ Human BCL11A One-Step ELISA Kit
Cat no : SE50110
Synonyms
B cell lymphoma/leukaemia 11A, B-cell CLL/lymphoma 11A, B-cell lymphoma/leukemia 11A, BCL-11A, COUP-TF-interacting protein 1
Validation Data Gallery
Product Information
| Product name | Speedy™ Human BCL11A One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 53335 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 89% | 75%-114% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.82 | 0.51 | 8.76 |
| 2 | 8 | 1.31 | 0.04 | 3.05 |
| 3 | 8 | 0.71 | 0.04 | 5.63 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.89 | 0.52 | 8.83 |
| 2 | 16 | 1.36 | 0.09 | 6.62 |
| 3 | 16 | 0.71 | 0.03 | 4.23 |
Background Information
B-Cell Lymphoma/Leukemia 11A(BCL11A) is a highly conserved zinc finger gene. It acts as a myeloid and B-cell proto-oncogene, and may play key roles in leukemogenesis and hematopoiesis. It is an essential factor in lymphopoiesis, and required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1. It is also involved in lymphoid malignancies through translocation or amplification events. It has recently been shown that BCL11A expression is inversely correlated with γ globin expression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B cell lymphoma/leukaemia 11A, B-cell CLL/lymphoma 11A, B-cell lymphoma/leukemia 11A, BCL-11A, COUP-TF-interacting protein 1 |
FAQ
1、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.