Speedy™ Human CD33 One-Step ELISA Kit
Cat no : SE50162
Synonyms
CD33 molecule, gp67, p67, Sialic acid-binding Ig-like lectin 3, SIGLEC 3
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD33 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 11.7 pg/mL |
| Range | 125-8000 pg/mL; 250-16000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 945 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 120% | 113%-127% |
| Cell lysate | 95% | 91%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.86 | 0.18 | 3.70 |
| 2 | 8 | 1.03 | 0.08 | 7.77 |
| 3 | 8 | 0.51 | 0.02 | 3.92 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.78 | 0.16 | 3.35 |
| 2 | 16 | 1.03 | 0.09 | 8.74 |
| 3 | 16 | 0.51 | 0.02 | 3.92 |
Background Information
CD33 is a type I transmembrane receptor glycoprotein that functions as an immune inhibitory receptor. It is expressed on myeloid cells, including monocytes, macrophages, and microglia, and plays a role in immune regulation and cell adhesion. CD33 is involved in the suppression of immune responses, particularly in the context of Alzheimer's disease, where it inhibits microglial uptake of amyloid beta. Additionally, CD33 is implicated in the regulation of inflammation and immune cell activation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD33 molecule, gp67, p67, Sialic acid-binding Ig-like lectin 3, SIGLEC 3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.