Human CD4 ELISA Kit
Cat no : KE00313
Synonyms
CD4, CD4 molecule, CD4mut
Validation Data Gallery
Product Information
KE00313 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD4 ELISA kit is to be used to detect and quantify protein levels of endogenous human CD4. The assay recognizes human CD4. An antibody specific for human CD4 has been pre-coated onto the microwells. The human CD4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD4 is added to detect the captured human CD4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 3.4 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 920 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 85% | 80%-102% |
| Cell lysate | 97% | 87%-120% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 470.0 | 16.3 | 3.5 |
| 2 | 20 | 120.5 | 7.3 | 6.1 |
| 3 | 20 | 29.0 | 1.2 | 4.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 472.0 | 23.2 | 4.9 |
| 2 | 24 | 111.7 | 4.9 | 4.4 |
| 3 | 24 | 25.2 | 2.0 | 8.0 |
Background Information
CD4 is a transmembrane glycoproteinof the immunoglobulin super-family (IgSF), containing four extracellular Ig-like domains, ahydrophobic transmembrane region and a 40-residue cytoplasmictail. CD4 is expressed T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells.CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interactionand it is involved in T cell differentiation, development and activation by interacting with antigen-presenting cells (APCs). The cytoplasmic tail of CD4 associates with the Lck kinase, which in turn activates the signaling components of the T cell receptor (TCR)-CD3 complexes.CD4 also serves as a receptor for human immunodeficiency virus (HIV) by binding to the viral envelope glycoprotein gp120.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD4, CD4 molecule, CD4mut |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.