Speedy™ Human CD45 One-Step ELISA Kit
Cat no : SE50103
Synonyms
EC:3.1.3.48, GP180, L CA, LCA, L-CA
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD45 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 8.7 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5788 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 95% | 90%-99% |
| Cell lysate | 88% | 87%-90% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,545.2 | 134.1 | 5.3 |
| 2 | 8 | 646.1 | 21.9 | 3.4 |
| 3 | 8 | 180.9 | 6.7 | 3.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,600.9 | 177.8 | 6.8 |
| 2 | 16 | 641.0 | 21.5 | 3.4 |
| 3 | 16 | 180.2 | 5.9 | 3.3 |
Background Information
CD45 is a transmembrane glycoprotein, expressed on almost all hematopoietic cells except for mature erythrocytes, and is an essential regulator of T and B cell antigen receptor-mediated activation. CD45 controls immune function by regulating lymphocyte survival, cytokine responses, and TCR signaling. Additionally, the soluble cytoplasmic tail of CD45 is a soluble factor in human plasma under steady-state conditions, which contributes to the maintenance of T-cell quiescence.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:3.1.3.48, GP180, L CA, LCA, L-CA |
FAQ
1、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.