Human CD8 Isolation Kit

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  Following cell separation, cell suspension was stained with FITC-CD45(F10-89-4), PE-CD3(UCHT1) and APC-CD8(OKT8) antibodies. All CD45+ cells are gated in the analysis. Left panel: CD3+CD8+ cells before selection. Right panel: CD3+CD8+ cells after selection. Human CD8 Isolation Kit is tested using PBMC from three donors.

Cat No. KMS303

Publications

1

Reactivity

Human

Application

Cell Separation

Conjugate

Magnetic Beads

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Product Information

CD8 is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within immune system. CD8 acts as a coreceptor with T cell receptor on T lymphocyte to recognize antigens displayed by antigen presenting cell in the context MHC II molecules.  10%-30% human lymphocytes are CD8 positive. Human CD8 Isolation Kit is designed to isolate untouched CD8+ T toxicity lymphocytes which contains a biotin labeled antibody cocktail and streptavidin conjugated magnetic beads. Through incubation of samples with biotin antibody cocktail followed by incubation of streptavidin beads, the magnetically labeled non CD8+ cells will be retained in the tube by magnetic separator. The untouched CD8+ T toxicity cells are collected in supernatant. Any downstream application can be performed on CD8+ cells after separation, such as Flow Cytometry and in vitro culture. 

Components

KMS303-10:  

  · MS303A-10: 300 µL Human CD8 Isolation biotin labeled human CD8 selection antibody cocktail (CD4, CD19, CD14, CD56, CD20, CD11b, CD16, CD36, CD235a, CD123, TCR γδ)

  · MS303B-10: 300µL streptavidin magnetic beads  

KMS303-100:  

  · MS303A-100: 3mL Human CD8 Isolation biotin labeled human CD8 selection antibody cocktail (CD4, CD19, CD14, CD56, CD20, CD11b, CD16, CD36, CD235a, CD123, TCR γδ)

  · MS303B-100: 3mL streptavidin magnetic beads  

Storage

2-8°C

Storage buffer

PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide

Reactivity

Human 

Recommend usage

30µL biotin labeled antibody cocktail and 30µL streptavidin beads for 1*10⁷ cells.

Cited in Article as

KMS303, Human CD8 Isolation Kit, Proteintech, IL, USA

Publications

Application	Title
	Cytotechnology; The oncogenic role of SPOCK1 in lung carcinoma by promoting immune evasion and its related mechanisms; Authors - Geni Lv