Human CD86 ELISA Kit
Cat no : KE00280
Synonyms
Activation B7 2 antigen, Activation B7-2 antigen, B7 2, B70, BU63
Validation Data Gallery
Product Information
KE00280 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD86 ELISA kit is to be used to detect and quantify protein levels of endogenous human CD86. The assay recognizes human CD86. An antibody specific for human CD86 has been pre-coated onto the microwells. The human CD86 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD86 is added to detect the captured human CD86 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD86 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 30.7 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 942 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 92% | 72%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4,930.1 | 143.9 | 2.9 |
| 2 | 20 | 1,107.4 | 28.6 | 2.6 |
| 3 | 20 | 305.1 | 23.2 | 7.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 4,989.5 | 155.1 | 3.1 |
| 2 | 24 | 1,126.1 | 51.6 | 4.6 |
| 3 | 24 | 277.6 | 20.3 | 7.3 |
Background Information
CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin (Ig) superfamily. CD86 is primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages. CD86 has strong structural similarity with another B7 family molecule, CD80 (B7-1). CD86 and CD80 are the ligands for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte antigen 4 (CTLA-4). Binding of CD86 or CD80 with CD28 antigen is a costimulatory signal for T cell activation, proliferation, and cytokine production. Binding of CD86 or CD80 with CTLA-4 negatively regulates T cell activation and diminishes the immune response. CD86 and CD80 bind to CTLA-4 with higher affinity than CD28. Defects in CTLA-4-mediated transendocytosis of CD86 are associated with autoimmunity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Activation B7 2 antigen, Activation B7-2 antigen, B7 2, B70, BU63 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.