Human CEACAM1 ELISA Kit
Cat no : KE00301
Synonyms
BGP, BGP 1, BGP1, BGPI, Biliary glycoprotein 1, CD66a, CEACAM1
Validation Data Gallery
Product Information
KE00301 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CEACAM1 ELISA kit is to be used to detect and quantify protein levels of endogenous CEACAM1. The assay recognizes human CEACAM1. An antibody specific for CEACAM1 has been pre-coated onto the microwells. The CEACAM1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for CEACAM1 is added to detect the captured CEACAM1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CEACAM1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.12 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 634 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 99% | 86%-112% |
| Cell culture supernatant | 90% | 85%-97% |
| cell lysate | 102% | 85%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 47.1 | 1.3 | 2.7 |
| 2 | 20 | 201.7 | 6.3 | 3.1 |
| 3 | 20 | 906.7 | 48.0 | 5.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 50.1 | 3.0 | 6.0 |
| 2 | 24 | 192.1 | 10.9 | 5.7 |
| 3 | 24 | 822.9 | 64.3 | 7.8 |
Background Information
CEACAM1, also known as BGP1 or CD66a, belongs to the CEA-related cell-adhesion molecule (CEACAM) subfamily, which is a part of the immunoglobulin superfamily. It plays a role in cell-cell adhesion, regulation of cell proliferation, insulin resistance, obesity, and vascular homeostasis. CEACAM1 is expressed by certain epithelial, endothelial, lymphoid, and myeloid cells. It is found on the surface of various tumors and is associated with tumor progression, metastasis and poor prognosis. CEACAM1 serves as a specific biomarker for Non-small cell lung cancer (NSCLC) and can be used for diagnosis and prognosis of melanoma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BGP, BGP 1, BGP1, BGPI, Biliary glycoprotein 1, CD66a, CEACAM1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.