Human COMP ELISA Kit
Cat no : KE00105
Synonyms
COMP, EDM1, EPD1, MED, PSACH, THBS5, Thrombospondin 5, TSP5
Validation Data Gallery
Product Information
KE00105 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The COMP ELISA kit is to be used to detect and quantify protein levels of endogenous COMP. The assay recognizes human COMP. An antibody specific for COMP has been pre-coated onto the microwells. The COMP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human COMP is added to detect the captured human COMP protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human COMP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 31.25 - 2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1311 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 104% | 76%-127% |
| Cell culture supernatants | 108% | 73%-122% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 818.1 | 70.1 | 8.6 |
| 2 | 20 | 194.2 | 18.3 | 9.4 |
| 3 | 20 | 51.5 | 4.6 | 9.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 874.3 | 81.0 | 9.3 |
| 2 | 24 | 206.0 | 20.5 | 9.9 |
| 3 | 24 | 44.2 | 4.4 | 9.9 |
Background Information
COMP is a secreted pentameric glycoprotein built from modular units and belongs to the thrombospondin protein family. COMP is expressed by hypertrophic chondrocytes and osteoblasts during endochondral ossification, and its expression is increased during fracture healing. COMP is involved in the assembly of collagen fibrils and other structural matrix components. COMP interacts with a number of cartilage ECM proteins, including fibronectin, collagen I, II IX, XII, and XIV, matrilins, as well as proteoglycans such as aggrecan and others.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | COMP, EDM1, EPD1, MED, PSACH, THBS5, Thrombospondin 5, TSP5 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.