Human CRP ELISA Kit
Cat no : KE00004
Synonyms
C reactive protein, CRP, PTX1
Validation Data Gallery
Product Information
KE00004 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CRP ELISA kit is to be used to detect and quantify protein levels of endogenous CRP. The assay recognizes human CRP. An antibody specific for CRP has been pre-coated onto the microwells. The CRP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for CRP is added to detect the captured CRP protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CRP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1401 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plamsa | 88% | 76%-97% |
| Cell culture supernatants | 115% | 106%-123% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2.05 | 0.16 | 7.6 |
| 2 | 20 | 3.15 | 0.28 | 8.8 |
| 3 | 20 | 7.45 | 0.68 | 9.2 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1.93 | 0.13 | 6.9 |
| 2 | 24 | 2.81 | 0.26 | 9.2 |
| 3 | 24 | 4.97 | 0.48 | 9.6 |
Background Information
C-reactive protein (CRP) is an acute-phase serum protein predominately synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C reactive protein, CRP, PTX1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Int J Mol Sci Interplay of Postprandial Triglyceride-Rich Lipoprotein Composition and Adipokines in Obese Adolescents | ||
Exp Gerontol Effect of intermittent hypoxic conditioning on inflammatory biomarkers in older adults. | ||
Dis Markers Identification of Protein Expression Changes in Hepatocellular Carcinoma through iTRAQ. | ||
Ann N Y Acad Sci Identification of maternal serum biomarkers for prenatal diagnosis of nonsyndromic orofacial clefts | ||
Cell Biochem Biophys DNA Receptor Toll-Like Receptor 9 Signaling Pathway Plays a Major Immunomodulatory Role in Neonatal Acute Lung Injury by Inhibiting Inflammatory Response and Oxidative Stress |