Human Coagulation Factor III/Tissue Factor ELISA Kit
Cat no : KE00400
Synonyms
CD142,Coagulation factor III,F3,Tissue factor,TF
Validation Data Gallery
Product Information
KE00400 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Coagulation Factor III/Tissue Factor ELISA kit is to be used to detect and quantify protein levels of endogenous human Coagulation Factor III/Tissue Factor. The assay recognizes human Coagulation Factor III/Tissue Factor. An antibody specific for human Coagulation Factor III/Tissue Factor has been pre-coated onto the microwells. The human Coagulation Factor III/Tissue Factor protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Coagulation Factor III/Tissue Factor is added to detect the captured human Coagulation Factor III/Tissue Factor protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Coagulation Factor III/Tissue Factor ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2152 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 83% | 81%-85% |
| Urine | 87% | 83%-90% |
| Cell lysate | 101% | 101%-102% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 238.9 | 4.4 | 1.8 |
| 2 | 20 | 56.6 | 1.0 | 1.7 |
| 3 | 20 | 13.0 | 0.5 | 3.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 242.4 | 6.3 | 2.6 |
| 2 | 24 | 58.7 | 1.4 | 2.5 |
| 3 | 24 | 13.9 | 1.0 | 7.5 |
Background Information
Tissue factor (TF),also named coagulation factor III or F3, is a transmembrane protein that functions as a high-affinity receptor for factor (F)VII and FVIIa. The TF-FVIIa complex is the primary initiator of blood coagulation and plays an essential role in hemostasis. TF is expressed on perivascular cells and epithelial cells at organ and body surfaces where it forms a hemostatic barrier. TF also provides additional hemostatic protection to vital organs, such as the brain, lung, and heart. Under pathological conditions, TF can trigger both arterial and venous thrombosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD142,Coagulation factor III,F3,Tissue factor,TF |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.