Human Cyclin E1 ELISA Kit
Cat no : KE00393
Synonyms
CCNE1, CCNE, Cyclin E, G1/S specific cyclin E1, G1/S-specific cyclin-E1
Validation Data Gallery
Product Information
KE00393 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Cyclin E1 ELISA kit is to be used to detect and quantify protein levels of endogenous human Cyclin E1. The assay recognizes human Cyclin E1. An antibody specific for human Cyclin E1 has been pre-coated onto the microwells. The human Cyclin E1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Cyclin E1 is added to detect the captured human Cyclin E1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Cyclin E1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 898 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 98% | 93%-103% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 8.67 | 0.40 | 4.60 |
| 2 | 20 | 2.07 | 0.08 | 3.95 |
| 3 | 20 | 0.51 | 0.04 | 7.58 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 9.40 | 0.32 | 3.40 |
| 2 | 24 | 2.29 | 0.09 | 3.78 |
| 3 | 24 | 0.60 | 0.04 | 6.32 |
Background Information
Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating cyclin-dependent kinase 2 (CDK2), regulates the G1-S phase transition of the mammalian cell division cycle.The transition from the quiescent state of the cell cycle into the phase of DNA synthesis is thought to be regulated by E-type cyclins Cyclin E1 (CCNE1) and Cyclin E2 (CCNE2) in complex with CDK2. During G1/S phase, cyclin dependent kinases (CDKs) (CDK4/6) is activated by cyclin D1, D2, or D3 to phosphorylate Rb, followed by phosphorylation by CDK2 in complex with cyclin E1 or cyclin E2. Cyclin E1 is highly related to cyclin E2, and both cyclin E1 and cyclin E2 are estrogen target genes that can facilitate anti-estrogen resistance and can be highly expressed in breast cancer. Amplified expressed cyclin E1 is a well-known tumorigenic factor and prognostic biomarker in several malignancies, such as osteosarcoma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CCNE1, CCNE, Cyclin E, G1/S specific cyclin E1, G1/S-specific cyclin-E1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.