Human EGF ELISA Kit
Cat no : KE00138
Synonyms
EGF, HOMG4, Pro epidermal growth factor, URG, Urogastrone
Validation Data Gallery
Product Information
KE00138 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The EGF ELISA kit is to be used to detect and quantify protein levels of endogenous EGF. The assay recognizes human EGF. An antibody specific for EGF has been pre-coated onto the microwells. The EGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for EGF is added to detect the captured EGF protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human EGF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 26.9 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1950 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 88% | 71%-110% |
| Human serum | 92% | 71%-112% |
| Cell culture supernatants | 101% | 80%-123% |
| Saliva | 102% | 79%-127% |
| Urine | 108% | 90%-125% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 531.9 | 18.2 | 3.4 |
| 2 | 20 | 2,019.4 | 63.1 | 3.1 |
| 3 | 20 | 7,679.1 | 273.4 | 3.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 583.4 | 37.5 | 6.4 |
| 2 | 24 | 2,306.4 | 182.9 | 7.9 |
| 3 | 24 | 7,538.8 | 608.2 | 8.1 |
Background Information
Epidermal growth factor (EGF) is a member of the epidermal growth factor superfamily. EGF preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. EGF binds to the EGF receptor on the surface of cells and mediates intrinsic phosphorylation of the receptor on tyrosine residues. It has been detected in nearly all body fluids, such as urine (urogastrone), saliva, milk and platelet-rich plasma. EGF plays important roles in multiple biological processes, such as the regulation of cell growth, proliferation, and differentiation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EGF, HOMG4, Pro epidermal growth factor, URG, Urogastrone |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Aging Cell Activation of epidermal growth factor receptor signaling mediates cellular senescence induced by certain pro-inflammatory cytokines. | ||
Exp Eye Res High-throughput transcriptional profiling combined with angiogenesis antibody array analysis in an orbital venous malformation cohort. | ||
Signal Transduct Target Ther Activation of Nerve Growth Factor signaling limits the response to lenvatinib in hepatocellular carcinoma | ||
Food Res Int Efficient expression of a cytokine combination in Saccharomyces cerevisiae for cultured meat production | ||
Indian J Hematol Blood Transfus Experimental Study on the Influence of Different Storage Conditions (Temperature, Duration) on Platelet Count, LDH, and Growth Factor Concentration in Platelet-Rich Plasma. |