Human EGFR ELISA Kit
Cat no : KE00294
Synonyms
EGFR,EGF R,EGF Receptor ,Epidermal growth factor r
Validation Data Gallery
Product Information
KE00294 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The EGFR ELISA kit is to be used to detect and quantify protein levels of endogenous EGFR. The assay recognizes human EGFR. An antibody specific for EGFR has been pre-coated onto the microwells. The EGFR protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for EGFR is added to detect the captured EGFR protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human EGFR ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant，Cell Lysate, Human milk |
| Assay type | Sandwich |
| Sensitivity | 0.029 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1956 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 84% | 77%-91% |
| Cell culture supernatant | 84% | 76%-90% |
| Cell Lysate | 84% | 71%-94% |
| Human milk | 90% | 78%-102% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4.82 | 0.21 | 4.35 |
| 2 | 20 | 1.15 | 0.03 | 2.20 |
| 3 | 20 | 0.28 | 0.01 | 3.13 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 4.74 | 0.25 | 5.18 |
| 2 | 24 | 1.14 | 0.04 | 3.54 |
| 3 | 24 | 0.29 | 0.01 | 3.09 |
Background Information
The epidermal growth factor receptor (EGFR) belongs to the ErbB family of receptor tyrosine kinases (RTKs) and exerts critical functions in epithelial cell physiology. EGFR is a receptor of tyrosine kinase (RTK). It is consisted of a C-terminus intracellular region that possesses the kinase activity, and an N-terminus extracellular ligand-binding site, a hydrophobic transmembrane domain. EGFR is frequently mutated and/or overexpressed in different types of human cancers and is the target of multiple cancer therapies currently adopted in the clinical practice. Mutations in this gene are associated with lung cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EGFR,EGF R,EGF Receptor ,Epidermal growth factor r |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
J Med Chem Erlotinib-Gold(I) Complex Induces Leukemia Cell DC Differentiation and Remodels the Immunosuppressive Microenvironment |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Shriya (Verified Customer) (08-29-2025) | EGFR ELISA kit, very sensitive and doesn’t require the whole day to perform
|