Speedy™ Human EMR2 One-Step ELISA Kit
Cat no : SE50123
Synonyms
ADGRE2, Adhesion G protein-coupled receptor E2, CD312, EGF like module receptor 2, EGF-like module receptor 2
Validation Data Gallery
Product Information
| Product name | Speedy™ Human EMR2 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 5.2 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 30817 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 94% | 75%-113% |
| Cell lysate | 94% | 84%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,485.4 | 76.6 | 5.2 |
| 2 | 8 | 362.5 | 12.9 | 3.6 |
| 3 | 8 | 191.1 | 7.4 | 3.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,455.7 | 64.1 | 4.4 |
| 2 | 16 | 359.6 | 10.1 | 2.8 |
| 3 | 16 | 192.2 | 10.8 | 5.6 |
Background Information
EMR2 (Epidermal Growth Factor-like module-containing Mucin-like Hormone Receptor 2), also known as ADGRE2, is a member of the adhesion-GPCR family that contains extracellular EGF-like domains. EMR2 contains a total of five tandem EGF-like domains and expresses similar protein isoforms consisting of various numbers of EGF-like domains as a result of alternative RNA splicing. EMR2 is restricted to myeloid cells including monocytes, macrophages, dendritic cells and granulocytes28 and its expression is highly regulated during monocyte/macrophage differentiation. The ligation of EMR2 could increase neutrophil adhesion, migration and anti-microbial mediator production and could enhance the systemic inflammation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ADGRE2, Adhesion G protein-coupled receptor E2, CD312, EGF like module receptor 2, EGF-like module receptor 2 |
FAQ
1、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.