Human FOLR1 ELISA Kit
Cat no : KE00115
Synonyms
Adult folate binding protein, FBP, Folate receptor 1, folate receptor 1 (adult), Folate receptor alpha, Folate receptor, adult, FOLR, FOLR1, FR alpha, KB cells FBP, MOv18
Validation Data Gallery
Product Information
KE00115 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human FOLR1 ELISA kit is to be used to detect and quantify protein levels of endogenous human . The assay recognizes human FOLR1. An antibody specific for human FOLR1 has been pre-coated onto the microwells. The human FOLR1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human FOLR1 is added to detect the captured human FOLR1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FOLR1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma ,Cell culture supernatants, Urine, Saliva, Human milk |
| Assay type | Sandwich |
| Sensitivity | 3.5 pg/mL |
| Range | 250 - 8000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2348 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human milk | 97% | 84%-112% |
| Cell culture supernatants | 106% | 82%-121% |
| Human saliva | 105% | 87%-123% |
| Human urine | 92% | 73%-118% |
| Human plasma | 89% | 75%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 494.9 | 14.5 | 2.9 |
| 2 | 20 | 944.2 | 22.4 | 2.4 |
| 3 | 20 | 3,824.7 | 110.3 | 2.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 482.8 | 21.0 | 4.3 |
| 2 | 24 | 933.4 | 50.6 | 5.4 |
| 3 | 24 | 3,760.1 | 133.3 | 3.5 |
Background Information
Folate receptor 1 (FOLR1), also known as folate receptor alpha or adult folate-binding protein (FBP), is a 38-kDa glycoprotein belonging to the folate receptor family. The receptor binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. FOLR1 expression is often limited to the apical surfaces of epithelium in the lung, kidney and choroid plexus but is differentially overexpressed in a variety of solid tumors such as ovarian cancer, non-small cell lung cancer, breast cancer, kidney cancer and high-grade osteosarcoma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Adult folate binding protein, FBP, Folate receptor 1, folate receptor 1 (adult), Folate receptor alpha, Folate receptor, adult, FOLR, FOLR1, FR alpha, KB cells FBP, MOv18 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.