Speedy™ Human Factor IX/PTC One-Step ELISA Kit
Cat no : SE50116
Synonyms
F9, Factor IX, Christmas factor, coagulation factor IX, Coagulation factor IXa heavy chain
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Factor IX/PTC One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 11.7 pg/mL |
| Range | 109.5-7000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2158 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 106% | 84%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 3,564.2 | 104.1 | 2.9 |
| 2 | 8 | 819.6 | 14.8 | 1.8 |
| 3 | 8 | 425.1 | 7.7 | 1.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 3,663.4 | 147.2 | 4.0 |
| 2 | 16 | 838.7 | 26.4 | 3.1 |
| 3 | 16 | 441.8 | 19.8 | 4.5 |
Background Information
Factor IX, also known as PTC, circulates as an inactive zymogen until the proteolytic release of its activation peptide allows it to assume the conformation of an active serine protease. Upon activation by Factor XIa or Factor VIIa/tissue factor complex, Factor IXa forms a complex with Factor VIIIa, calcium ions, and phospholipids on the surface of activated platelets. This complex, known as the tenase complex, efficiently activates Factor X to Factor Xa, leading to thrombin generation and fibrin clot formation. Deficiencies or mutations in Factor IX result in Hemophilia B, an X-linked recessive bleeding disorder.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | F9, Factor IX, Christmas factor, coagulation factor IX, Coagulation factor IXa heavy chain |
FAQ
1、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.